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基本信息

質粒圖譜和多克隆位點信息

注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

Note: This is negative control vector. It contains a negative control siRNA template (55 base pairs) between the BamH I and Hind III sites.

載體描述

哺乳動物干擾載體，siRNA表達載體。 

載體序列

LOCUS       Exported                4944 bp ds-DNA     circular SYN 21-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pSilencer4.1-CMV-Neo

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4944)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Thursday, Mar 21, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..4944

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     primer_bind     333..350

                     /label=5'primer

     primer_bind     379..395

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar

                     variants"

     misc_feature    401..520

                     /label=MCS

     misc_feature    463..517

                     /label=siRNA

                     /note="a negative control siRNA template (55 base pairs)

                     between the BamH I and Hind III sites. "

     promoter        complement(536..739)

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early

                     promoter"

     primer_bind     complement(558..575)

                     /label=3'primer

     enhancer        740..1043

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        complement(1165..1183)

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     promoter        complement(1195..1213)

                     /label=SP6 promoter

                     /note="promoter for bacteriophage SP6 RNA polymerase"

     primer_bind     complement(1233..1249)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    1257..1273

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(1281..1311)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    1326..1347

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(1635..2223)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(2394..3254)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     polyA_signal    3284..3405

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     CDS             complement(3581..4375)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     promoter        complement(4442..4758)

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4456..4591

                     /label=SV40 ori

                     /note="SV40 origin of replication"

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctagcggcc gcatacaaaa

      421 aaccaacaca cagatccaat gaaaataaaa gatcctttat taagcttact accgttgtta

      481 taggtgtctc ttgaacacct ataacaacgg tagtggatcc acggttcact aaaccagctc

      541 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt

      601 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt

      661 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt

      721 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag

      781 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat

      841 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag

      901 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg

      961 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc

     1021 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg

     1081 taattgatta ctattaataa ctaagatctg gtaccttgaa ttcatgcttc tcctcccttt

     1141 agtgagggta attctctctc tctccctata gtgagtcgta ttaattcctt ctcttctata

     1201 gtgtcaccta aatcgttgca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt

     1261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt

     1321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg

     1381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg

     1441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg

     1501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat

     1561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc

     1621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc

     1681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga

     1741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt

     1801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg

     1861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc

     1921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg

     1981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc

     2041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg

     2101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa aaaaaccacc

     2161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct

     2221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt

     2281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa

     2341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa

     2401 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc

     2461 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct

     2521 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca

     2581 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt

     2641 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt

     2701 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc

     2761 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc

     2821 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt

     2881 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact

     2941 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc

     3001 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt

     3061 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg

     3121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct

     3181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa

     3241 tgttgaatac tcatactctt cctttttcaa tattcagaca tgataagata cattgatgag

     3301 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat

     3361 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa

     3421 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc

     3481 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg

     3541 cgtcgcttgg tcggtcattt cgcgaacccc agagtcccgc tcagaagaac tcgtcaagaa

     3601 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc

     3661 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct

     3721 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt

     3781 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg

     3841 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt

     3901 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat

     3961 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg

     4021 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc

     4081 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag

     4141 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt

     4201 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca

     4261 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata

     4321 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa

     4381 acgatcctca tcctgtctct tgatcagatc cgaaaatgga tatacaagct cccgggagct

     4441 ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg

     4501 cagaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat

     4561 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg

     4621 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc

     4681 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc

     4741 ctggggactt tccacaccaa tattattgaa gcatttatca gggttattgt ctcatgagcg

     4801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc

     4861 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata

     4921 ggcgtatcac gaggcccttt cgtc

//