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基本信息

質粒圖譜和多克隆位點信息 

注意：

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如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

pCAMBIA系列載體是常用的植物表達雙元載體，載體的骨架是pUC18。其中 pCAMBIA1300， pCAMBIA2300均無gus報告基因，pCAMBIA系列載體均含有卡那霉素抗性基因。pCAMBIA2300特點：大腸桿菌篩選抗性卡那霉素，植物篩選抗性：新霉素。

載體序列

LOCUS       pCAMBIA2300                8740 bp ds-DNA     circular SYN 25-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pCAMBIA2300

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 8740)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Monday, Mar 25, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..8740

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             1219..1848

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     CDS             2282..3349

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 RepA

                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA

                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR

                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR

                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL

                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA

                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV

                     MRYRNLIEGEASAGS"

     rep_origin      3415..3609

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

     misc_feature    3953..4093

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(4279..4867)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(4954..5748)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     misc_feature    6173..6197

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     polyA_signal    6275..6449

                     /label=CaMV poly(A) signal

                     /note="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(6506..7303)

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418

                     (Geneticin(R))"

                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR

                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL

                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH

                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI

                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     promoter        complement(7366..8041)

                     /label=CaMV 35S promoter (enhanced)

                     /note="cauliflower mosaic virus 35S promoter with a

                     duplicated enhancer region"

     protein_bind    8232..8253

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     promoter        8268..8298

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    8306..8322

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     primer_bind     8330..8346

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     CDS             8342..8575

                     /codon_start=1

                     /gene="lacZ fragment"

                     /product="LacZ-alpha fragment of beta-galactosidase"

                     /label=lacZ-alpha

                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR

                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"

     misc_feature    8356..8412

                     /label=MCS

                     /note="pUC18/19 multiple cloning site"

     primer_bind     complement(8416..8432)

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar

                     variants"

     misc_feature    8635..8659

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

ORIGIN

        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct

       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca

      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt

      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca

      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga

      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca

      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg

      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca

      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg

      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg

      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg

      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga

      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga

      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg

      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac

      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac

      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt

     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg

     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt

     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca

     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc

     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg

     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa

     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc

     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg

     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc

     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa

     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag

     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac

     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc

     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta

     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca

     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc

     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca

     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa

     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc

     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc

     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga

     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga

     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg

     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc

     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc

     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg

     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca

     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact

     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa

     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga

     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt

     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga

     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga

     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct

     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct

     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg

     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc

     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt

     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca

     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga

     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa

     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa

     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc

     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg

     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc

     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc

     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg

     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg

     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca

     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca

     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa

     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg

     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg

     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa

     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg

     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc

     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc

     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc

     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg

     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc

     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga

     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc

     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac

     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg

     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc

     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata

     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata

     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt

     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa

     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt

     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc

     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc

     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc

     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag

     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag

     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac

     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc

     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta

     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct

     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc

     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg

     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca

     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt

     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag

     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat

     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga

     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt

     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg

     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag

     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg

     6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg

     6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga

     6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc

     6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca

     6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg

     6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga

     6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat

     6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt

     6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg

     6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca

     7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc

     7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg

     7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg

     7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag

     7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct

     7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc

     7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg

     7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga

     7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg

     7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca

     7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg

     7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact

     7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc

     7801 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg

     7861 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc

     7921 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca

     7981 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc

     8041 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc

     8101 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt

     8161 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag

     8221 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg

     8281 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc

     8341 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag

     8401 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt

     8461 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga

     8521 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag

     8581 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag

     8641 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa

     8701 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg

//