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基本信息

質粒圖譜和多克隆位點信息

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載體說明

pTRV2-EGFP: Tobacco rattle virus (TRV) based cDNA clone was placed between the duplicated CaMV 35S promoter and the nopaline synthase terminator in a T-DNA vector. EGFP was introduced in pTRV2 before MCS.

載體序列

LOCUS       Exported               10380 bp ds-DNA     circular SYN 26-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pTRV2-EGFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 10380)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Tuesday, Mar 26, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..10380

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    1..1172

                     /label=TRV RNA2 5'

                     /note="TRV strain ppk20 RNA2 5'-sequence"

     CDS             1173..1892

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     misc_feature    1893..2356

                     /label=TRV RNA2 5'

                     /note="TRV strain ppk20 RNA2 5'-sequence"

     misc_feature    2364..2425

                     /label=MCS

     misc_feature    2426..2820

                     /label=TRV RNA2 3'

                     /note="TRV strain ppk20 RNA2 3'-sequence"

     CDS             2881..2898

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     terminator      2933..3185

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

     misc_feature    3207..3231

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

     CDS             4531..5160

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     CDS             5594..6661

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 RepA

                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA

                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR

                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR

                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL

                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA

                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV

                     MRYRNLIEGEASAGS"

     rep_origin      6727..6921

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

     misc_feature    7265..7405

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(7591..8179)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(8266..9060)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     misc_feature    9485..9509

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     misc_feature    9625..10380

                     /label=2-chi-CaMV 35S

     primer_bind     10312..10331

                     /label=35S primer

     promoter        10335..10380

                     /label=minimal CaMV 35S promoter

                     /note="minimal 35S promoter from cauliflower mosaic virus"

ORIGIN

        1 ataaaacatt gcacctatgg tgttgccctg gctggggtat gtcagtgatc gcagtagaat

       61 gtactaattg acaagttgga gaatacggta gaacgtcctt atccaacaca gcctttatcc

      121 ctctccctga cgaggttttt gtcagtgtaa tatttctttt tgaactatcc agcttagtac

      181 cgtacgggaa agtgactggt gtgcttatct ttgaaatgtt actttgggtt tcggttcttt

      241 aggttagtaa gaaagcactt gtcttctcat acaaaggaaa acctgagacg tatcgcttac

      301 gaaagtagca atgaaagaaa ggtggtggtt ttaatcgcta ccgcaaaaac gatggggtcg

      361 ttttaattaa cttctcctac gcaagcgtct aaacggacgt tggggttttg ctagtttctt

      421 tagagaaaac tagctaagtc tttaatgtta tcattagaga tggcataaat ataatacttg

      481 tgtctgctga taagatcatt ttaatttgga cgattagact tgttgaacta caggttactg

      541 aatcacttgc gctaatcaac atgggagata tgtacgatga atcatttgac aagtcgggcg

      601 gtcctgctga cttgatggac gattcttggg tggaatcagt ttcgtggaaa gatctgttga

      661 agaagttaca cagcataaaa tttgcactac agtctggtag agatgagatc actgggttac

      721 tagcggcact gaatagacag tgtccttatt caccatatga gcagtttcca gataagaagg

      781 tgtatttcct tttagactca cgggctaaca gtgctcttgg tgtgattcag aacgcttcag

      841 cgttcaagag acgagctgat gagaagaatg cagtggcggg tgttacaaat attcctgcga

      901 atccaaacac aacggttacg acgaaccaag ggagtactac tactaccaag gcgaacactg

      961 gctcgacttt ggaagaagac ttgtacactt attacaaatt cgatgatgcc tctacagctt

     1021 tccacaaatc tctaacttcg ttagagaaca tggagttgaa gagttattac cgaaggaact

     1081 ttgagaaagt attcgggatt aagtttggtg gagcagctgc tagttcatct gcaccgcctc

     1141 cagcgagtgg aggtccgata cgtcctaatc ccatggtgag caagggcgag gagctgttca

     1201 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg

     1261 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca

     1321 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc

     1381 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc

     1441 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc

     1501 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg

     1561 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca

     1621 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc

     1681 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg

     1741 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca

     1801 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga

     1861 tcactctcgg catggacgag ctgtacaagt aaggatttaa ggacgtgaac tctgttgaga

     1921 tctctgtgaa attcagaggg tgggtgatac catattcact gatgccatta gcgacatcta

     1981 aatagggcta attgtgacta atttgaggga atttccttta ccattgacgt cagtgtcgtt

     2041 ggtagcattt gagtttcgca atgcacgaat tacttaggaa gtggcttgac gacactaatg

     2101 tgttattgtt agataatggt ttggtggtca aggtacgtag tagagtccca catattcgca

     2161 cgtatgaagt aattggaaag ttgtcagttt ttgataattc actgggagat gatacgctgt

     2221 ttgagggaaa agtagagaac gtatttgttt ttatgttcag gcggttcttg tgtgtcaaca

     2281 aagatggaca ttgttactca aggaagcacg atgagcttta ttattacgga cgagtggact

     2341 tagattctgt gagtaaggtt accgaattct ctagaaggcc tccatgggga tccggtaccg

     2401 agctcacgcg tctcgaggcc cgggcatgtc ccgaagacat taaactacgg ttctttaagt

     2461 agatccgtgt ctgaagtttt aggttcaatt taaacctacg agattgacat tctcgactga

     2521 tcttgattga tcggtaagtc ttttgtaatt taattttctt tttgatttta ttttaaattg

     2581 ttatctgttt ctgtgtatag actgtttgag atcggcgttt ggccgactca ttgtcttacc

     2641 ataggggaac ggactttgtt tgtgttgtta ttttatttgt attttattaa aattctcaac

     2701 gatctgaaaa agcctcgcgg ctaagagatt gttggggggt gagtaagtac ttttaaagtg

     2761 atgatggtta caaaggcaaa aggggtaaaa cccctcgcct acgtaagcgt tattacgccc

     2821 gtctgtactt atatcagtac actgacgagt ccctaaagga cgaaacggga gaacgctagc

     2881 caccaccacc accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc

     2941 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat

     3001 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt

     3061 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga

     3121 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact

     3181 agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa

     3241 aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc

     3301 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac

     3361 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg

     3421 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt

     3481 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt

     3541 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg

     3601 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt

     3661 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc

     3721 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg

     3781 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag

     3841 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca

     3901 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca

     3961 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg

     4021 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg

     4081 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg

     4141 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg

     4201 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc

     4261 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc

     4321 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa

     4381 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag

     4441 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg

     4501 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa

     4561 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg

     4621 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg

     4681 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg

     4741 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg

     4801 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac

     4861 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat

     4921 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt

     4981 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag

     5041 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag

     5101 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga

     5161 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga

     5221 gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc

     5281 gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc

     5341 aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga

     5401 gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa

     5461 gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca

     5521 ggcgtaagcg gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag

     5581 gaatcggcgt gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga

     5641 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga

     5701 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca

     5761 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga

     5821 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt

     5881 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct

     5941 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta

     6001 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg

     6061 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg

     6121 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa

     6181 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt

     6241 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc

     6301 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa

     6361 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt

     6421 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac

     6481 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa

     6541 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg

     6601 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta

     6661 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct

     6721 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc

     6781 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat

     6841 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa

     6901 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc

     6961 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc

     7021 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc

     7081 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg

     7141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt

     7201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc

     7261 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc

     7321 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg

     7381 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg

     7441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc

     7501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag

     7561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca

     7621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca

     7681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg

     7741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag

     7801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt

     7861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca

     7921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg

     7981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt

     8041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc

     8101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg

     8161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg

     8221 gaacgaaaac tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc

     8281 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa

     8341 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat

     8401 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc

     8461 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc

     8521 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc

     8581 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc

     8641 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag

     8701 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc

     8761 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg

     8821 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt

     8881 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag

     8941 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt

     9001 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat

     9061 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac

     9121 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct

     9181 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa

     9241 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc

     9301 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta

     9361 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg

     9421 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg

     9481 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat

     9541 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcctaggcca ccatgttggg

     9601 cccggcgcgc caagcttgca tgcctgcagg tcaacatggt ggagcacgac actctcgtct

     9661 actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag acttttcaac

     9721 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatcg

     9781 aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat aaaggaaagg

     9841 ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca cccacgagga

     9901 acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgatg

     9961 gtcaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca

    10021 gaagaccaga gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga

    10081 ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga agatggcttc

    10141 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc taccgacagt

    10201 ggtcccaaag atggaccccc acccacgagg aacatcgtgg aaaaagaaga cgttccaacc

    10261 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa

    10321 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg

//