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基本信息

質粒圖譜和多克隆位點信息

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載體描述

植物過表達載體，帶EYFP報告基因，由pGreenII-62-SK改造而來，融合表達EYFP蛋白。 

載體序列

LOCUS       Exported                4058 bp ds-DNA     circular SYN 27-MAR-2019

DEFINITION  .

ACCESSION   .

VERSION     .

KEYWORDS    pGreenII 62-SK-EYFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4058)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Wednesday, March 27, 2019 from SnapGene 3.2.1

FEATURES             Location/Qualifiers

     source          1..4058

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    542..564

                     /note="LB T-DNA repeat"

                     /note="left border repeat from nopaline C58 T-DNA

                     (truncated)"

                     /note="color: #ffe4c4"

     promoter        638..982

                     /note="CaMV 35S promoter"

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

                     /note="color: #ffffff; direction: RIGHT"

     misc_feature    992..1084

                     /note="MCS"

                     /note="pBluescript multiple cloning site"

                     /note="color: #99ccff"

     primer_bind     1016..1032

                     /note="SK primer"

                     /note="common sequencing primer, one of multiple similar

                     variants"

                     /note="color: #a020f0; direction: RIGHT"

     primer_bind     complement(1066..1082)

                     /note="KS primer"

                     /note="common sequencing primer, one of multiple similar

                     variants"

                     /note="color: #a020f0; direction: LEFT"

     CDS             1085..1804

                     /codon_start=1

                     /product="enhanced YFP"

                     /note="EYFP"

                     /note="mammalian codon-optimized"

                     /note="This feature has 3 segments:

                        1: 1085 .. 1087 / #e1ff2f

                        2: 1088 .. 1090 / #e1ff2f / 1a

                        3: 1091 .. 1804 / #e1ff2f"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     polyA_signal    1864..2040

                     /note="CaMV poly(A) signal"

                     /note="cauliflower mosaic virus polyadenylation signal"

                     /note="color: #a6acb3"

     misc_feature    2050..2074

                     /note="RB T-DNA repeat"

                     /note="right border repeat from nopaline C58 T-DNA"

                     /note="color: #ffe4c4"

     rep_origin      complement(2165..2753)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

                     /note="color: #ffff00"

     CDS             complement(2924..3739)

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /note="KanR"

                     /note="confers resistance to kanamycin in bacteria or G418

                     (Geneticin(R)) in eukaryotes"

                     /note="color: #ccffcc"

                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG

                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK

                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA

                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI

                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

     rep_origin      4030..407

                     /note="pSa ori"

                     /note="origin of replication from bacterial plasmid pSa"

                     /note="color: #ffff00"

ORIGIN

        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc

       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg

      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg

      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc

      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat

      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac

      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc

      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa

      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc

      541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa

      601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa

      661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag

      721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc

      781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg

      841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg

      901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa

      961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag

     1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct

     1081 cgagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct

     1141 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac

     1201 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc

     1261 caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat

     1321 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat

     1381 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac

     1441 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg

     1501 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa

     1561 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct

     1621 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa

     1681 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat

     1741 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa

     1801 gtaaggtacc aattcggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc

     1861 tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata

     1921 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa

     1981 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc

     2041 cactagcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg tttgtttgag

     2101 atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg

     2161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga

     2221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg

     2281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg

     2341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc

     2401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg

     2461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca

     2521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt

     2581 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag

     2641 ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg

     2701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc

     2761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt

     2821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt

     2881 ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattagaaa aactcatcga

     2941 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa

     3001 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct

     3061 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt

     3121 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg

     3181 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat

     3241 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa

     3301 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga

     3361 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga

     3421 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa

     3481 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat

     3541 ctgtaacaac attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg

     3601 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt

     3661 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt

     3721 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta

     3781 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa

     3841 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc

     3901 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct

     3961 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc

     4021 aggcgatccc catccaacag cccgccgtcg agcgggct

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