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基本信息

質粒圖譜和多克隆位點信息

注意：

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載體描述

敲除載體，以pcDNA3.3-TOPO為骨架，插入人cas9蛋白編碼序列。

載體序列

LOCUS       Exported                9553 bp ds-DNA     circular SYN 10-APR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pcDNA3.1-hCas9

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 9553)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Wednesday, Apr 10, 2019 from SnapGene Viewer 4.3.6

FEATURES             Location/Qualifiers

     source          1..9553

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        50..429

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        430..633

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early

                     promoter"

     regulatory      742..751

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation

                     of translation (Kozak, 1987)"

     CDS             748..4851

                     /codon_start=1

                     /product="Cas9 (Csn1) endonuclease from the Streptococcus

                     pyogenes Type II CRISPR/Cas system"

                     /label=Cas9

                     /note="generates RNA-guided double strand breaks in DNA"

                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK

                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES

                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK

                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL

                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ

                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR

                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL

                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG

                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY

                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD

                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER

                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM

                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR

                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY

                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP

                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH

                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL

                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT

                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK

                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI

                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE

                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD

                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL

                     DATLIHQSITGLYETRIDLSQLGGD"

     CDS             4864..4884

                     /codon_start=1

                     /product="nuclear localization signal of SV40 (simian virus

                     40) large T antigen"

                     /label=SV40 NLS

                     /translation="PKKKRKV"

     polyA_signal    4983..5031

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      5233..5661

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow

                     indicates direction of (+) strand synthesis"

     promoter        5675..6004

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      5855..5990

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             6071..6865

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    7041..7162

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     primer_bind     complement(7211..7227)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    7235..7251

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(7259..7289)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    7304..7325

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(7613..8201)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(8372..9232)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(9233..9337)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta

       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag

      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc

      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga

      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat

      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc

      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct

      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca

      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat

      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg

      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg

      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg

      721 actctagagg atcgaaccct tgccaccatg gacaagaagt actccattgg gctcgatatc

      781 ggcacaaaca gcgtcggctg ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa

      841 ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat tggcgccctc

      901 ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc acggcgcaga

      961 tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa tgagatggct

     1021 aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga ggaggataaa

     1081 aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta ccatgaaaag

     1141 tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa ggctgacttg

     1201 cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt cctcatcgag

     1261 ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact ggttcagact

     1321 tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc caaagcaatc

     1381 ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca gctccctggg

     1441 gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct gacccccaac

     1501 tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa agacacctac

     1561 gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga cctttttttg

     1621 gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt gaacacggag

     1681 atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca ccaccaagac

     1741 ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa ggaaattttc

     1801 ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag ccaggaggaa

     1861 ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga gctgctggta

     1921 aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg aagcatcccc

     1981 caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga tttctacccc

     2041 tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat accctactat

     2101 gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa atcagaagag

     2161 accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc ccagtccttc

     2221 atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct tcctaaacac

     2281 tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa atacgtcaca

     2341 gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat cgtggacctc

     2401 ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta tttcaaaaag

     2461 attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa cgcatccctg

     2521 ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga caatgaggag

     2581 aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga tagggagatg

     2641 attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat gaaacagctc

     2701 aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa tgggatccga

     2761 gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt tgccaaccgg

     2821 aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat ccagaaagca

     2881 caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc aggtagccca

     2941 gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt caaagtaatg

     3001 ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca aactacccag

     3061 aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat aaaagaactg

     3121 gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa tgagaagctc

     3181 tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact ggacatcaat

     3241 cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa agatgattct

     3301 attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga taacgtcccc

     3361 tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa cgccaaactg

     3421 atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct gtctgagttg

     3481 gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac caagcacgtg

     3541 gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa actgattcga

     3601 gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa ggactttcag

     3661 ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta cctgaatgca

     3721 gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt tgtttacgga

     3781 gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga aataggcaag

     3841 gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac cgagattaca

     3901 ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga aacaggagaa

     3961 atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc catgccgcag

     4021 gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga aagtatcctc

     4081 ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc caagaaatac

     4141 ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa agtggagaaa

     4201 gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat catggagcga

     4261 tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa agaggtcaaa

     4321 aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa cggccggaaa

     4381 cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact gccctctaaa

     4441 tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc tcccgaagat

     4501 aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga gatcatcgag

     4561 caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga taaggtgctt

     4621 tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa cattatccac

     4681 ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga caccaccata

     4741 gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat tcatcagtca

     4801 attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga cagcagggct

     4861 gaccccaaga agaagaggaa ggtgtgaaag ggttcgatcc ctaccggtta gtaatgagtt

     4921 taaacggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat

     4981 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg

     5041 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat

     5101 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc

     5161 tcgcagccaa cgtcggggcg gcaggccctg ccatagcaga tctgcgcagc tggggctcta

     5221 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc

     5281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt

     5341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag

     5401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt

     5461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt

     5521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt

     5581 cttttgattt ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt

     5641 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc

     5701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag

     5761 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta

     5821 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc

     5881 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc

     5941 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg

     6001 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg

     6061 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga

     6121 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt

     6181 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct

     6241 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg

     6301 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt

     6361 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc

     6421 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc

     6481 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga

     6541 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg

     6601 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat

     6661 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg

     6721 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc

     6781 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta

     6841 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag

     6901 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg

     6961 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc

     7021 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca

     7081 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt

     7141 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg

     7201 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca

     7261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca

     7321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc

     7381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt

     7441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact

     7501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag

     7561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata

     7621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc

     7681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg

     7741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc

     7801 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg

     7861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc

     7921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga

     7981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg

     8041 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa

     8101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg

     8161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt

     8221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat

     8281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct

     8341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta

     8401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa

     8461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac

     8521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa

     8581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag

     8641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg

     8701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag

     8761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg

     8821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc

     8881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat

     8941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata

     9001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa

     9061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca

     9121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc

     9181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc

     9241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg

     9301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac

     9361 ctgacgtcga cggatcggga gatctcccga tcccctatgg tcgactctca gtacaatctg

     9421 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga

     9481 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa

     9541 gaatctgctt agg

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