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基本信息

質粒圖譜和多克隆位點信息
注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。  

載體描述
乳酸乳球菌通用表達質粒pMG36e是一個經典的人工構建的組成型表達載體，是以乳酸乳球菌乳脂亞種蛋白酶基因的轉錄和翻譯信號為基礎構建而成的。它包含一個強啟動子，能夠在多種細菌中表達外源蛋白。已用于研究細菌素作用機制，乳酸菌基因工程菌株的改造以及口服疫苗的開發等，應用領域十分廣泛，已成為乳酸菌基因工程研究的重要工具質粒之一。

載體序列
LOCUS       Exported                3611 bp ds-DNA     circular SYN 09-MAR-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Mar 9, 2019 from SnapGene Viewer 4.3.4
            http://bt-lab.cn
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             227..925
                     /codon_start=1
                     /label=repA
                     /note="Replication gene A"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             1463..2197
                     /codon_start=1
                     /product="Erythromycin"
                     /label=Emr
                     /note="Erythromycin"
                     /translation="LLIK*FIAIEKR*ELFKANIV*IVNSCMF*GIVKLIFCKYFLVFF
                     VNPFHNEIIILLFIFV*YS*FFST*SDK*AIHFRFRMKIFSWNHT*YRNINFCH*K*CQ
                     *AFCI**SFSKPVFHD*INLISYTIKNNFAYYIRTYVIRYITIYFIGLVFRKFKLQYIL
                     V*NLEIIVINKFIFCSFA*FMVYFNGSYEITPLY*FKGKMAFS*ADFKDIIMFI*SYIC
                     HYFIYIMF*SNKVLTVFYIFLVH"
     promoter        2474..2652
                     /label=P32
     misc_feature    2652..2671
                     /label=P32 F
     misc_feature    2687..2844
                     /label=MCS
     misc_feature    complement(2784..2801)
                     /label=P32 R
     CDS             complement(3215..3226)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
ORIGIN
        1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
       61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat
      121 agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag
      181 aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg ctattaaaaa
      241 tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta atgattggaa
      301 agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg atatggacga
      361 aaaaaaagat aaagatacat ggaatagtag tgatgttata cgaaatggaa agcactataa
      421 aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag aaagcgttag
      481 gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga tacttgatta
      541 tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg ctaagaataa
      601 acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg accgctatat
      661 aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata tagtggatga
      721 ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg gagcggagtt
      781 tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta gcgcctttag
      841 attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa aggttcttga
      901 tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc tgaaaatgag
      961 gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag agaaatggaa
     1021 gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata aataaaagcc
     1081 ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta atagcaatac
     1141 aattgcaata aacaaaatga tcgacctcgg gacccctatc tagcgaactt ttagaaaaga
     1201 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
     1261 aagctagctt tttagacatc taaatctagg tactaaaaca attcatccag taaaatataa
     1321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     1381 tgttcttccc cgatcgaccc gattcacaaa aaataggcac acgaaaaaca agttaaggga
     1441 tgcagtttat gcatccctta acttacttat taaataattt atagctattg aaaagagata
     1501 agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt aaggaattgt
     1561 taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc ataacgaaat
     1621 aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt aatctgataa
     1681 gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata cttaatatag
     1741 aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt aataatcttt
     1801 tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca aaaacaattt
     1861 tgcgtattat atccgtactt atgttataag gtatattacc atatatttta taggattggt
     1921 ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta tcgtgatcaa
     1981 caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca gttacgaaat
     2041 tacacctctt tactaattca agggtaaaat ggccttttcc tgagccgatt tcaaagatat
     2101 tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt tttgaagtaa
     2161 taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt aatttggtta
     2221 tatgaatttt gcttattaac gattcattat aaccacttat tttttgtttg gttgataatg
     2281 aactgtgctg attacaaaaa tactaaaaat gcccatattt tttcctcctt ataaaattag
     2341 tataattata gcacggtcga tcttctatat aaaagatata ttatcttatc agtattgtca
     2401 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
     2461 aaatatgggt cgatcgaatt cggtcctcgg gatatgataa gattaatagt tttagctatt
     2521 aatctttttt tatttttatt taagaatggc ttaataaagc ggttactttg gatttttgtg
     2581 agcttggact agaaaaaaac ttcacaaaat gctatactag gtaggtaaaa aaatattcgg
     2641 aggaattttg aaatggcaat cgtttcagca gaaaaattcg taattcgagc tcgcccgggg
     2701 atcgatcctc tagagtcgac ctgcaggcat gcaagcttgc aaagtctgaa aacgaaggtg
     2761 gcagctgccg ttgaagcggc caagacagtt ggtaaaggcg acggtacaac cggtactagc
     2821 gacaaaggcg gcggtcaagg taccccggcg ctacgatatt tggagttgag gttcaaagtc
     2881 aaatggtact gatgaccggt aaaatttaat attttgaacc ttgcttaggc agctgacttc
     2941 acattgttga gatcagctgc cttttgctta tagttcattg agtagaaacg gttctgttgc
     3001 gaagtttgaa aatcaaacgc aagctcgatt ttttattaaa acgtctcaaa atcgtttctg
     3061 agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg
     3121 tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc tgttagatta
     3181 tgaaagccga tgactgaatg aaataataag cgcagcgccc ttctatttcg gttggaggag
     3241 gctcaaggga gtatgaggga atgaaattcc ctcatgggtt tgattttaaa aattgcttgc
     3301 aattttgccg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt tggaaaaaaa
     3361 tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag tgccgagtgc
     3421 caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttaagg ggtttttcaa
     3481 tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat ttaagtagct
     3541 ttattgttgt ttttatgatt acaaagtgat acactaactt tataaaatta tttgattgga
     3601 gttttttaaa t
//