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基本信息

質粒圖譜和多克隆位點信息

注意：

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如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

pTargetF是一個大腸桿菌gRNA表達質粒，CRISPR載體。

載體序列

LOCUS       Exported                2118 bp ds-DNA     circular SYN 16-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 2118)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Saturday, Mar 16, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..2118

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             124..915

                     /codon_start=1

                     /gene="aadA"

                     /product="aminoglycoside adenylyltransferase (Murphy,

                     1985)"

                     /product="confers resistence to spectinomycin and

                     streptomycin"

                     /label=SmR

                     /note="confers resistance to spectinomycin and

                     streptomycin"

                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH

                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK

                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF

                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP

                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"

     rep_origin      1090..1678

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     primer_bind     1844..1863

                     /label=MoClo-F

     promoter        1926..1960

                     /label=J23119(SpeI) promoter

                     /note="bacterial promoter (Registry of Standard Biological

                     Parts BBa_J23119) modified to end with an SpeI site"

     CDS             1981..2056

                     /codon_start=1

                     /product="guide RNA scaffold for the Streptococcus pyogenes

                                          CRISPR/Cas9 system"

                     /label=gRNA scaffold

                     /note="guide RNA scaffold for the Streptococcus pyogenes

                     CRISPR/Cas9 system"

                     /translation="VLELEIAS*NKASPLST*KSGTESV"

     misc_feature    2064..2099

                     /label=MCS

                     /note="Multi clone site"

ORIGIN

        1 tcgagttcat gtgcagctcc ataagcaaaa ggggatgata agtttatcac caccgactat

       61 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat

      121 gtcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc

      181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat

      241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat

      301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag

      361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg

      421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca

      481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga

      541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa

      601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg

      661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc

      721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag

      781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg

      841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag

      901 gtagtcggca aataagatgc cgctcgccag tcgattggct gagctcatga agttcctatt

      961 ccgaagttcc gcgaacgcgt aaaggatcta ggtgaagatc ctttttgata atctcatgac

     1021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa

     1081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc

     1141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt

     1201 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg

     1261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc

     1321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt

     1381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga

     1441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct

     1501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg

     1561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca

     1621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa

     1681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt

     1741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga

     1801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga

     1861 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatgctg

     1921 gatccttgac agctagctca gtcctaggta taatactagt catcgccgca gcggtttcag

     1981 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt

     2041 ggcaccgagt cggtgctttt tttgaattct ctagagtcga cctgcagaag cttagatcta

     2101 ttaccctgtt atccctac

//