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基本信息

質粒圖譜和多克隆位點信息

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載體描述

藻類表達載體，由pPHA-T1改造而來。

載體序列

LOCUS       Exported                4806 bp ds-DNA     circular SYN 11-APR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pPHA-T1-EGFP

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4806)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Thursday, Apr 11, 2019 from SnapGene Viewer 4.3.6

FEATURES             Location/Qualifiers

     source          1..4806

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        1..142

                     /label=fcpA promoter

                     /note="promoter, fcp(fucoxanthin, chlorophyll a/c-binding

                     protein) from phaeodactylum tricornutum"

     primer_bind     221..240

                     /label=PfcpA-for

     primer_bind     375..394

                     /label=PTV-For

     misc_feature    443..478

                     /label=MCS

     CDS             479..1198

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     primer_bind     complement(524..545)

                     /label=pEGFPN3

     misc_feature    1199..1228

                     /label=3'MCS

     misc_feature    1229..1558

                     /label=fcpa terminator

                     /note="terminator, fcpA"

     primer_bind     complement(1243..1262)

                     /label=PTV-Rev

     promoter        1559..1803

                     /label=fcpB promoter

     CDS             1804..2178

                     /codon_start=1

                     /gene="Sh ble from Streptoalloteichus hindustanus"

                     /product="antibiotic-binding protein"

                     /label=BleoR

                     /note="confers resistance to bleomycin, phleomycin, and

                     Zeocin(TM)"

                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD

                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR

                     EFALRDPAGNCVHFVAEEQD"

     misc_feature    2179..2420

                     /label=fcpA terminator

     promoter        complement(2430..2448)

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     rep_origin      complement(2706..3294)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(3465..4325)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4326..4430)

                     /gene="bla"

                     /label=AmpR promoter

     promoter        4776..4794

                     /label=SP6 promoter

                     /note="promoter for bacteriophage SP6 RNA polymerase"

ORIGIN

        1 gggctgcagg acgcaatgga ggattatcac cgcaaaaatg aacttcgaaa aaaactttcg

       61 agcgaccatg gaaaaggagg atcagattca gattacaaca gtggattgct ctggtagcaa

      121 atatcttctg ctagattggc tcatggtcgg ttttggacgt tcgaagctca ccgtcaaaag

      181 aaacaaaaga gaagaatgac gtcttcgtga cgtagaatct acgactgtac tcggatctgg

      241 gaaatgaatt gactcacggt cttcttcgag tcctgttaca ggcccttggt ccgaaccccc

      301 acacgatttt tgcaccaaag atttgcttca atttgctgga tgttttgact gcaagatcag

      361 ctggcctagc aagagtgctc gtgttgcttc gtcgggaatc cctacgaatt tcagttctgc

      421 acaaatttgt ctgccgtttc gagaattcga tatcatcgac taattcgagc tcggtaccat

      481 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg

      541 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg

      601 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct

      661 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca

      721 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt

      781 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt

      841 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa

      901 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg

      961 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga

     1021 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta

     1081 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct

     1141 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaatc

     1201 tagagtcgac ctgcaggcat gcaagcttca gaagcgtgct atcgaactca accagggacg

     1261 tgcggcacaa atgggcatcc ttgctctcat ggtgcacgaa cagttgggag tctctatcct

     1321 tccttaaaaa tttaattttc attagttgca gtcactccgc tttggtttca cagtcaggaa

     1381 taacactagc tcgtcttcac catggatgcc aatctcgcct attcatggtg tataaaagtt

     1441 caacatccaa agctagaact tttggaaaga gaaagaatat ccgaataggg cacggcgtgc

     1501 cgtattgttg gagtggacta gcagaaagtg aggaaggcac aggatgagtt ttctcgagac

     1561 ataccttcag cgtcgtcttc actgtcacag tcaactgaca gtaatcgttg atccggagag

     1621 attcaaaatt caatctgttt ggacctggat aagacacaag agcgacatcc tgacatgaac

     1681 gccgtaaaca gcaaatcctg gttgaacacg tatccttttg ggggcctccg ctacgacgct

     1741 cgctccagct ggggcttcct tactatacac agcgcgcata tttcacggtt gccagatgtc

     1801 aagatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg

     1861 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc

     1921 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg

     1981 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg

     2041 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag

     2101 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg

     2161 gccgaggagc aggactgaac cttccttaaa aatttaattt tcattagttg cagtcactcc

     2221 gctttggttt cacagtcagg aataacacta gctcgtcttc accatggatg ccaatctcgc

     2281 ctattcatgg tgtataaaag ttcaacatcc aaagctagaa cttttggaaa gagaaagaat

     2341 atccgaatag ggcacggcgt gccgtattgt tggagtggac tagcagaaag tgaggaaggc

     2401 acaggatgag ttttctcgag gccggtctcc ctatagtgag tcgtattaat ttcgataagc

     2461 caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg

     2521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg

     2581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga

     2641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg

     2701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag

     2761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc

     2821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg

     2881 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt

     2941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc

     3001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc

     3061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg

     3121 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca

     3181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc

     3241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat

     3301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt

     3361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt

     3421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc

     3481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc

     3541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata

     3601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg

     3661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc

     3721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct

     3781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa

     3841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt

     3901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca

     3961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac

     4021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca

     4081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt

     4141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc

     4201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca

     4261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata

     4321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc

     4381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc

     4441 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat

     4501 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga

     4561 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa

     4621 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca

     4681 tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac

     4741 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta tagaaccaga

     4801 tccccc

//