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基本信息

質粒圖譜和多克隆位點信息

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載體描述

pMV261為分枝桿菌表達載體。

載體序列

LOCUS       Exported                4488 bp ds-DNA     circular SYN 30-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pMV261

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4488)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Saturday, Mar 30, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..4488

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             113..928

                     /codon_start=1

                     /gene="aph(3')-Ia"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin in bacteria or G418

                     (Geneticin(R)) in eukaryotes"

                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG

                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK

                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA

                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI

                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"

     rep_origin      1260..1848

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     rep_origin      1849..2002

                     /label=pUC ori

     rep_origin      2007..3902

                     /label=mycobacterial origin

                     /note="mycobacterial origin region from pAL5000"

     primer_bind     3607..3624

                     /label=PWM261AF

     promoter        3931..4312

                     /label=Hsp60 promoter

                     /note="groEL promoter"

     primer_bind     4295..4312

                     /label=Hsp60 primer

     misc_feature    4313..4381

                     /label=MCS

                     /note="multiple cloning site"

     terminator      4398..4444

                     /label=rrnB T1 terminator

                     /note="transcription terminator T1 from the E. coli rrnB

                     gene"

ORIGIN

        1 acaaagcgac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc

       61 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca

      121 tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt

      181 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt

      241 gtatgggaag ccccatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa

      301 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac

      361 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg

      421 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc

      481 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag

      541 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc

      601 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca

      661 taatcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa

      721 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc

      781 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt

      841 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt

      901 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca

      961 gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac ttttgctgag

     1021 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag

     1081 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact

     1141 ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc ttcttcacga

     1201 ggcagacctc actagttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1381 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

     1441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

     1621 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

     1681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

     1741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

     1801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

     1861 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

     1921 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

     1981 cgcagccgaa cgaccgagcg caacgcgtga gcccaccagc tccgtaagtt cgggtgctgt

     2041 gtggctcgta cccgcgcatt caggcggcag ggggtctaac gggtctaagg cggcgtgtac

     2101 ggccgccaca gcggctctta gcggcccgga aacgtcctcg aaacgacgca tgtgttcctc

     2161 ctggttggta caggtggttg ggggtgctcg gctgtcgctg gtgtttcatc atcagggctc

     2221 gacgggagag cgggggagtg tgcagttgtg gggtggcccc tcagcgaaat atctgacttg

     2281 gagctcgtgt cggaccatac accggtgatt aatcgtggtt tattatcaag cgtgagccac

     2341 gtcgccgacg aatttgagca gctctggctg ccgtactggt ccctggcaag cgacgatctg

     2401 ctcgagggga tctaccgcca aagccgcgcg tcggccctag gccgccggta catcgaggcg

     2461 aacccaacag cgctggcaaa cctgctggtc gtggacgtag accatccaga cgcagcgctc

     2521 cgagcgctca gcgcccgggg gtcccatccg ctgcccaacg cgatcgtggg caatcgcgcc

     2581 aacggccacg cacacgcagt gtgggcactc aacgcccctg ttccacgcac cgaatacgcg

     2641 cggcgtaagc cgctcgcata catggcggcg tgcgccgaag gccttcggcg cgccgtcgat

     2701 ggcgaccgca gttactcagg cctcatgacc aaaaaccccg gccacatcgc ctgggaaacg

     2761 gaatggctcc actcagatct ctacacactc agccacatcg aggccgagct cggcgcgaac

     2821 atgccaccgc cgcgctggcg tcagcagacc acgtacaaag cggctccgac gccgctaggg

     2881 cggaattgcg cactgttcga ttccgtcagg ttgtgggcct atcttcccgc cctcatgcgg

     2941 atctacctgc cgacccggaa cgtggacgga ctcggccgcg cgatctatgc cgagtgccac

     3001 gcgcgaaacg ccgaatttcc gtgcaacgac gtgtgtcccg gaccgctacc ggacagcgag

     3061 gtccgcgcca tcgccaacag catttggcgt tggatcacaa ccaagtcgcg catttgggcg

     3121 gacgggatcg tggtctacga ggccacactc agtgcgcgcc atgcggccat ctcgcggaag

     3181 ggcgcagcag cgcgcacggc ggcgagcaca gttgcgcggc gcgcaaagtc cgcgtcagcc

     3241 atggaggcat tgctatgagc gacggctaca gcgacggcta cagcgacggc tacaactggc

     3301 agccgactgt ccgcaaaaag cggcgcgtga ccgccgccga aggcgctcga atcaccggac

     3361 tatccgaacg ccacgtcgtc cggctcgtgg cgcaggaacg cagcgagtgg ttcgccgagc

     3421 aggctgcacg ccgcgaacgc atccgcgcct atcacgacga cgagggccac tcttggccgc

     3481 aaacggccaa acatttcggg ctgcatctgg acaccgttaa gcgactcggc tatcgggcga

     3541 ggaaagagcg tgcggcagaa caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc

     3601 caccgctgtt ctaacgcaat tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt

     3661 tgcaacgggt cggacaggta aaagtcctgg tagacgctag ttttctggtt tgggccatgc

     3721 ctgtctcgtt gcgtgtttcg ttgcgtccgt tttgaatacc agccagacga gacggggttc

     3781 tacgaatctt ggtcgatacc aagccatttc cgctgaatat cgtggagctc accgccagaa

     3841 tcggtggttg tggtgatgta cgtggcgaac tccgttgtag tgcttgtggt ggcatccgtg

     3901 gcgcggccgc ggtaccagat ctttaaatct agaggtgacc acaacgacgc gcccgctttg

     3961 atcggggacg tctgcggccg accatttacg ggtcttgttg tcgttggcgg tcatgggccg

     4021 aacatactca cccggatcgg agggccgagg acaaggtcga acgaggggca tgacccggtg

     4081 cggggcttct tgcactcggc ataggcgagt gctaagaata acgttggcac tcgcgaccgg

     4141 tgagtcgtag gtcgggacgg tgaggccagg cccgtcgtcg cagcgagtgg cagcgaggac

     4201 aacttgagcc gtccgtcgcg ggcactgcgc ccggccagcg taagtagcgg ggttgccgtc

     4261 acccggtgac ccccggtttc atccccgatc cggaggaatc acttcgcaat ggccaagaca

     4321 attgcggatc cagctgcaga attcgaagct tatcgatgtc gacgtagtta actagcgtac

     4381 gatcgactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt

     4441 ttatctgttg tttgtccggc catcatggcc gcggtgatca gctagcca

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