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基本信息

質粒圖譜和多克隆位點信息

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載體描述

FLAG標簽含8個氨基酸殘基(DYKDDDDK)；pCMV-Tag 2 A,B,C的差別僅在于讀碼框（閱讀框）處。

XL1-Blue感受態細胞能夠利用藍白斑篩選進行重組質粒的鑒定，是使用質粒或Lambda載體進行常規克隆的優良的克隆菌株。

載體序列

LOCUS       pCMV-Tag 2C 4325 bp DNA SYN

DEFINITION  pCMV-Tag 2C

ACCESSION   

KEYWORDS    

SOURCE      

  ORGANISM  other sequences; artificial sequences; vectors.

FEATURES             Location/Qualifiers

     source          1..4325

                     /organism="pCMV-Tag 2C"

                     /mol_type="other DNA"

     promoter        16..568

                     /label="CMV_immearly_promoter"

     misc_feature    71..358

                     /label="CAG_enhancer"

     misc_feature    525..545

                     /label="CMV_fwd_primer"

     promoter        526..595

                     /label="CMV_promoter"

     promoter        620..639

                     /label="T3_promoter"

     misc_feature    complement(755..771)

                     /label="pBluescriptKS_primer"

     promoter        complement(827..845)

                     /label="T7_promoter"

     misc_feature    902..961

                     /label="SV40_3_splice"

     terminator      1012..1240

                     /label="SV40_PA_terminator"

     misc_feature    1210..1229

                     /label="EBV_rev_primer"

     rep_origin      complement(1379..1685)

                     /label="f1_origin"

     promoter        1764..1792

                     /label="AmpR_promoter"

     misc_feature    complement(1858..1878)

                     /label="pBABE_3_primer"

     misc_feature    complement(1864..2079)

                     /label="SV40_enhancer"

     promoter        1876..2144

                     /label="SV40_promoter"

     rep_origin      2043..2120

                     /label="SV40_origin"

     misc_feature    2105..2124

                     /label="SV40pro_F_primer"

     CDS             2227..3021

                     /label="ORF frame 1"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR

                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL

                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE

                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY

                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"

     gene            2230..3018

                     /label="NeoR/KanR (variant)"

                     /gene="NeoR/KanR (variant)"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR

                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL

                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE

                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY

                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"

     CDS             complement(2536..3072)

                     /label="ORF frame 3"

                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA

                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA

                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ

                     PPHCISHDGYFLGRSKVR*"

     terminator      3196..3465

                     /label="TK_PA_terminator"

                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA

                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA

                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ

                     PPHCISHDGYFLGRSKVR*"

     rep_origin      3613..4232

                     /label="pBR322_origin"

                     /translation="MAGWASLGRSFRTPESRSEELVKKAIEGDALRIGSGDTVKHEEA

                     VSPFAAKFFSNITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILA

                     VGHARLEPGEQFGWREPLMFFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQ

                     PPHCISHDGYFLGRSKVR*"

ORIGIN

    1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga

   61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg

  121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg

  181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca

  241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc

  301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc

  361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc

  421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa

  481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag

  541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta

  601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc

  661 gcggtggcgg ccgccaccat ggattacaag gatgacgacg ataagaagcc cgggcggatc

  721 ccccgggctg caggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc

  781 cggtacctta attaattaag gtaccaggta agtgtaccca attcgcccta tagtgagtcg

  841 tattacaatt cactcgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaga

  901 tccaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga

  961 ttcacagtcc caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa

 1021 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc

 1081 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata

 1141 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc

 1201 attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt

 1261 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag

 1321 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt

 1381 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga

 1441 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg

 1501 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct

 1561 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc

 1621 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt

 1681 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct

 1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga

 1801 taaatgcttc aataatattg aaaaaggaag aatcctgagg cggaaagaac cagctgtgga

 1861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa

 1921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca

 1981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc

 2041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt

 2101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag

 2161 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg

 2221 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg

 2281 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg

 2341 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat

 2401 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca

 2461 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg

 2521 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat

 2581 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa

 2641 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg

 2701 gacgaagaac atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg

 2761 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg

 2821 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat

 2881 caggacatag cgttggctac ccgtgatatt gctgaagaac ttggcggcga atgggctgac

 2941 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc

 3001 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc

 3061 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg

 3121 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt

 3181 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa

 3241 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc

 3301 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt

 3361 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag

 3421 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat

 3481 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc

 3541 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag

 3601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct

 3661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac

 3721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc

 3781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg

 3841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt

 3901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt

 3961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc

 4021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca

 4081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata

 4141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg

 4201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct

 4261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta

 4321 ccgcc

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