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基本信息

質粒圖譜和多克隆位點信息

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載體描述

BIFC質粒，黃色熒光標簽融合表達載體， 帶有雙35S啟動子及增強子，cEYFP位于目的基因C端。

載體序列

LOCUS       Exported                4075 bp ds-DNA     circular SYN 19-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pSAT6A-cEYFP-N1

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4075)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Tuesday, Mar 19, 2019 from SnapGene Viewer 4.3.4

            http://bt-lab.cn/

COMMENT     Alias: pE3086

FEATURES             Location/Qualifiers

     source          1..4075

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     primer_bind     379..395

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar

                     variants"

     promoter        421..762

                     /label=CaMV 35S promoter

                     /note="CaMV 35S promoter"

     promoter        768..1089

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     5'UTR           1090..1181

                     /note="5'UTR of Tobacco Etch Virus"

     5'UTR           1190..1320

                     /note="5' UTR from tobacco etch virus;"

                     /note="translational enhancer"

     misc_feature    1321..1377

                     /label=MCS

                     /note="multiple cloning site"

     misc_feature    1380..1577

                     /label=cEYFP

                     /note="carboxyl terminus of EYFP"

     primer_bind     complement(1483..1500)

                     /label=cEYFP R primer

     terminator      1596..1806

                     /note="CaMV 35S terminator"

     primer_bind     complement(1854..1870)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    1878..1894

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(1902..1932)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    1947..1968

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(2256..2844)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(3015..3875)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase,  amp resistance"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3876..3980)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct

      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag

      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa

      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg

      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct

      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag

      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca

      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga

      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc

      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata

      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac

     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt

     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc

     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa

     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta

     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag

     1321 agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcctgg

     1381 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc

     1441 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga

     1501 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg

     1561 acgagctgta caagtgaaga tccacctagt ctagagtccg caaaaatcac cagtctctct

     1621 ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag

     1681 ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat

     1741 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc

     1801 agtgacgcgg ccgcacccat aatacccata atagctgttt gccagtaatc atggtcatag

     1861 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc

     1921 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc

     1981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa

     2041 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg

     2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg

     2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag

     2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac

     2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga

     2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt

     2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc

     2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc

     2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta

     2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat

     2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca

     2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct

     2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt

     2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct

     2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc

     2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa

     3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta

     3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc

     3121 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat

     3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta

     3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt

     3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt

     3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg

     3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc

     3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc

     3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg

     3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga

     3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta

     3721 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct

     3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag

     3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga

     3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat

     3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc

     4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc

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