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基本信息

質粒圖譜和多克隆位點信息

注意：

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如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

The pGL4.17[luc2/Neo] Vector(a–e)encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomolous transcription and has a synthetic reporter gene, which has been codon optimized for mammalian expression.

The pGL4.17[luc2/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.

載體序列

LOCUS       Exported                5599 bp ds-DNA     circular SYN 01-APR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pGL4.17

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5599)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Monday, Apr 1, 2019 from SnapGene Viewer 4.3.6

FEATURES             Location/Qualifiers

     source          1..5599

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    14..70

                     /label=MCS

     regulatory      94..103

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation

                     of translation (Kozak, 1987)"

     CDS             100..1752

                     /codon_start=1

                     /product="firefly luciferase"

                     /label=luciferase

                     /note="synthetic luc2 version of the luciferase gene"

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"

     primer_bind     complement(134..151)

                     /label=GLP4

     polyA_signal    1796..1917

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     promoter        2111..2468

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2319..2454

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2499..3293

                     /codon_start=1

                     /product="aminoglycoside phosphotransferase"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3318..3366

                     /note="synthetic polyadenylation signal"

     rep_origin      complement(3693..4281)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(4481..5341)

                     /codon_start=1

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     polyA_signal    5446..5494

                     /note="synthetic polyadenylation signal"

     misc_feature    5508..5599

                     /label=pause site

                     /note="RNA polymerase II transcriptional pause signal from

                     the human alpha-2 globin gene"

                     /note="synthetic poly?A?signal/transcriptional pause site

                     for background reduction"

     primer_bind     5548..5567

                     /label=pGL4.17 F

ORIGIN

        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc

       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt

      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac

      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc

      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg

      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag

      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac

      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc

      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa

      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc

      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac

      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc

      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc

      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc

      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc

      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg

      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc

     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg

     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc

     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc

     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc

     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct

     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac

     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac

     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc

     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg

     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca

     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc

     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag

     1741 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga

     1801 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt

     1861 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac

     1921 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa

     1981 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtttgc gtattgggcg

     2041 ctcttccgct gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt

     2101 tagctacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa

     2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa

     2221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca

     2281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca

     2341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg

     2401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct

     2461 tttgcaaaaa gctcgattct tctgacacta gcgccaccat gatcgaacaa gacggcctcc

     2521 atgctggcag tcccgcagct tgggtcgaac gcttgttcgg gtacgactgg gcccagcaga

     2581 ccatcggatg tagcgatgcg gccgtgttcc gtctaagcgc tcaaggccgg cccgtgctgt

     2641 tcgtgaagac cgacctgagc ggcgccctga acgagcttca agacgaggct gcccgcctga

     2701 gctggctggc caccaccggc gtaccctgcg ccgctgtgtt ggatgttgtg accgaagccg

     2761 gccgggactg gctgctgctg ggcgaggtcc ctggccagga tctgctgagc agccaccttg

     2821 cccccgctga gaaggtttct atcatggccg atgcaatgcg gcgcctgcac accctggacc

     2881 ccgctacctg ccccttcgac caccaggcta agcatcggat cgagcgtgct cggacccgca

     2941 tggaggccgg cctggtggac caggacgacc tggacgagga gcatcagggc ctggcccccg

     3001 ctgaactgtt cgcccgactg aaagcccgca tgccggacgg tgaggacctg gttgtcacac

     3061 acggagatgc ctgcctccct aacatcatgg tcgagaatgg ccgcttctcc ggcttcatcg

     3121 actgcggtcg cctaggagtt gccgaccgct accaggacat cgccctggcc acccgcgaca

     3181 tcgctgagga gcttggcggc gagtgggccg accgcttctt agtcttgtac ggcatcgcag

     3241 ctcccgacag ccagcgcatc gccttctacc gcttgctcga cgagttcttt taatgatcta

     3301 gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt

     3361 tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac ccagtcagct

     3421 ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca

     3481 tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac tgactcgctg

     3541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta

     3601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc

     3661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag

     3721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac

     3781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc

     3841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt

     3901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc

     3961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga

     4021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta

     4081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta

     4141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga

     4201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg

     4261 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag

     4321 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc

     4381 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact

     4441 tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc ttgatcagtg

     4501 aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga ctccccgtcg

     4561 tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca atgatgccgc

     4621 gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca gggagggccg

     4681 agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc tgctgtcgtg

     4741 atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc attgctactg

     4801 gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt tcccagcggt

     4861 caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc ttagggcctc

     4921 cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg gcagcactac

     4981 acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc gagtactcaa

     5041 ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg gcgtctatac

     5101 gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg aatcgttctt

     5161 cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata tagcccactc

     5221 ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg tgtgcaaaaa

     5281 caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt tggatgctca

     5341 tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac gtctctcaag

     5401 gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa aatatcttta

     5461 ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc

     5521 catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca

     5581 ggtgccagaa catttctct

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