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基本信息

質粒圖譜和多克隆位點信息
注意：

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載體描述

將目的片段兩端加酶切位點，正反連接到一個intron的兩端，表達后成為一個shRNA，達到RNAi的效果。

載體序列

LOCUS       Exported               10193 bp ds-DNA     circular SYN 22-MAR-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    pCAMBIA1300-35S-X

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 10193)

  AUTHORS   BT-Lab

  TITLE     Direct Submission

  JOURNAL   Exported Friday, Mar 22, 2019 from SnapGene Viewer 4.3.4

FEATURES             Location/Qualifiers

     source          1..10193

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     protein_bind    103..124

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     promoter        315..992

                     /label=CaMV 35S promoter (enhanced)

                     /note="cauliflower mosaic virus 35S promoter with a

                     duplicated enhancer region"

     CDS             1060..2085

                     /codon_start=1

                     /gene="aph(4)-Ia"

                     /product="aminoglycoside phosphotransferase from E. coli"

                     /label=HygR

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

                     K"

     polyA_signal    2125..2299

                     /label=CaMV poly(A) signal

                     /note="cauliflower mosaic virus polyadenylation signal"

     misc_feature    2377..2401

                     /label=LB T-DNA repeat

                     /note="left border repeat from nopaline C58 T-DNA"

     CDS             2826..3620

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label=KanR

                     /note="confers resistance to kanamycin"

                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM

                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED

                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT

                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA

                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"

     rep_origin      3707..4295

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     misc_feature    4481..4621

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      4965..5159

                     /label=pVS1 oriV

                     /note="origin of replication for the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

     CDS             complement(5225..6292)

                     /codon_start=1

                     /product="replication protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 RepA

                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA

                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR

                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR

                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL

                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA

                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV

                     MRYRNLIEGEASAGS"

     CDS             complement(6726..7355)

                     /codon_start=1

                     /product="stability protein from the Pseudomonas plasmid

                     pVS1 (Heeb et al., 2000)"

                     /label=pVS1 StaA

                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR

                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP

                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI

                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"

     misc_feature    8655..8679

                     /label=RB T-DNA repeat

                     /note="right border repeat from nopaline C58 T-DNA"

     primer_bind     8882..8898

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar

                     variants"

     promoter        9413..9758

                     /label=CaMV 35S promoter

                     /note="strong constitutive promoter from cauliflower mosaic

                     virus"

     primer_bind     9701..9721

                     /label=CaMV 35S primer

     misc_feature    9767..9786

                     /label=MCS

     misc_feature    9767..9786

                     /label=MCS

     misc_feature    9787..9898

                     /label=INTRON

     misc_feature    9899..9922

                     /label=MCS

     misc_feature    9905..9922

                     /label=MCS

     terminator      9933..10185

                     /label=NOS terminator

                     /note="nopaline synthase terminator and poly(A) signal"

ORIGIN

        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa

       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac

      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca

      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc

      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa

      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc

      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac

      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt

      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg

      541 tcttcaaagc aagtggattg atgtgataac atggtggagc acgacactct cgtctactcc

      601 aagaatatca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg

      661 gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg

      721 acagtagaaa aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc

      781 gttcaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc

      841 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc

      901 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagaccttcc tctatataag

      961 gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct acaaatctat

     1021 ctctctcgag ctttcgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc

     1081 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag

     1141 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc

     1201 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt

     1261 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggagtttag cgagagcctg

     1321 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa

     1381 ctgcccgctg ttctacaacc ggtcgcggag gctatggatg cgatcgctgc ggccgatctt

     1441 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg

     1501 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac

     1561 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac

     1621 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac

     1681 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac

     1741 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc

     1801 tacttcgagc ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc

     1861 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct

     1921 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca

     1981 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat

     2041 agtggaaacc gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg

     2101 gatctgtcga tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa

     2161 gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta

     2221 tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc

     2281 cagtactaaa atccagatcc cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt

     2341 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc

     2401 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg

     2461 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct

     2521 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg

     2581 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac

     2641 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt

     2701 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc

     2761 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat

     2821 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct

     2881 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg

     2941 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac

     3001 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact

     3061 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct

     3121 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca

     3181 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc

     3241 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact

     3301 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa

     3361 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga

     3421 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt

     3481 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg

     3541 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt

     3601 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgtgagtttt

     3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt

     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt

     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga

     3841 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag

     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata

     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg

     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga

     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca

     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa

     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt

     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac

     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt

     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga

     4441 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc

     4501 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg

     4561 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc

     4621 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc

     4681 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc

     4741 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg

     4801 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc

     4861 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg

     4921 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc

     4981 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct

     5041 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa

     5101 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag

     5161 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg

     5221 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga

     5281 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga

     5341 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt

     5401 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt

     5461 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt

     5521 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt

     5581 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct

     5641 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg

     5701 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc

     5761 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc

     5821 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca

     5881 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt

     5941 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa

     6001 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga

     6061 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg

     6121 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc

     6181 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca

     6241 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc

     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt

     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt

     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca

     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt

     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt

     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg

     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca

     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg

     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc

     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca

     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc

     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat

     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac

     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg

     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct

     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca

     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac

     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat

     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac

     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg

     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg

     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga

     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt

     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg

     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg

     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc

     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg

     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc

     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca

     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag

     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg

     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt

     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc

     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat

     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac

     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga

     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg

     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac

     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt ctaataaacg

     8641 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga

     8701 aggcgggaaa cgacaatctg atccaagctc aagctgctct agcattcgcc attcaggctg

     8761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa

     8821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt

     8881 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tccccagatt agccttttca

     8941 atttcagaaa gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca

     9001 agacgatcta cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag

     9061 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca

     9121 agatcagaag tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag

     9181 taatagagat tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa

     9241 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga

     9301 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac

     9361 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt

     9421 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact

     9481 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag

     9541 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca

     9601 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat

     9661 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt

     9721 cctctatata aggaagttca tttcatttgg agagaacacg ggggacggat cctgacgtcg

     9781 actagtagag ggtaattaag caaaacttat ccaaaactaa acattttact attattttga

     9841 cctttttatt ccacttttct tagacaatga tttaacctcg taatcaattg ttaggatttc

     9901 tagaggtacc agatctgagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt

     9961 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt

    10021 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta

    10081 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa

    10141 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttc

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