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必騰預構載體定制最高150元/個

基本信息

質粒圖譜和多克隆位點信息

注意：

客戶在我公司選擇構建載體服務項目時，請自行提供母源載體。

如要使用本公司保存的母源載體時，本公司不能保證所有母源載體完全符合客戶預期，也不承擔由此產生的任何責任。

載體描述

由pTOL2-MCS改造而來，加入mCherry標簽，可用于啟動子活性檢測。

載體序列

LOCUS       Exported                3753 bp ds-DNA     circular SYN 03-JUL-2019

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 3753)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Jul 3, 2019 from SnapGene Viewer 4.2.6

            http://www.snapgene.com

FEATURES             Location/Qualifiers

     source          1..3753

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    7..205

                     /label=TOL2-L

     misc_feature    206..273

                     /label=MCS

     regulatory      274..283

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation

                     of translation (Kozak, 1987)"

     CDS             280..990

                     /codon_start=1

                     /product="monomeric derivative of DsRed fluorescent protein

                     (Shaner et al., 2004)"

                     /label=mCherry

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

                     EGRHSTGGMDELYK"

     polyA_signal    997..1118

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     misc_feature    1133..1328

                     /label=TOL2-R

     primer_bind     complement(1344..1360)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar

                     variants"

     protein_bind    1368..1384

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to

                     inhibit transcription in E. coli. This inhibition can be

                     relieved by adding lactose or

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     promoter        complement(1392..1422)

                     /label=lac promoter

                     /note="promoter for the E. coli lac operon"

     protein_bind    1437..1458

                     /label=CAP binding site

                     /bound_moiety="E. coli catabolite activator protein"

                     /note="CAP binding activates transcription in the presence

                     of cAMP."

     rep_origin      complement(1746..2334)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of

                     replication"

     CDS             complement(2505..3365)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(3366..3470)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt

       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa

      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa

      181 agtacttatt ttttggagat cacttagatc taactgcaga acgtacgaac ccgggaaacg

      241 cgttctagag tcgacggtac cggatccggg cccgccacca tggtgagcaa gggcgaggag

      301 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg

      361 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag

      421 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc

      481 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac

      541 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc

      601 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg

      661 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc

      721 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag

      781 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag

      841 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc

      901 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc

      961 accggcggca tggacgagct gtacaagtag ctcgacaact tgtttattgc agcttataat

     1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat

     1081 tctagttgtg gtttgtccaa actcatcaat gtatcttact cgagaaaagc ttaaacaaga

     1141 atctctagtt ttctttcttg cttttacttt tacttcctta atactcaagt acaattttaa

     1201 tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt

     1261 gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt

     1321 acacctctga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc

     1381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga

     1441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg

     1501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg

     1561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg

     1621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga

     1681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg

     1741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag

     1801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc

     1861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg

     1921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt

     1981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc

     2041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc

     2101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg

     2161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca

     2221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc

     2281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat

     2341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt

     2401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt

     2461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc

     2521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc

     2581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata

     2641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg

     2701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc

     2761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct

     2821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa

     2881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt

     2941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca

     3001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac

     3061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca

     3121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt

     3181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc

     3241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca

     3301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata

     3361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc

     3421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc

     3481 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat

     3541 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga

     3601 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa

     3661 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca

     3721 tcagagcaga ttgtactgag agtgcaccat atg

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